Journal: iScience

Article Title: Uhrf1 governs the proliferation and differentiation of muscle satellite cells

doi: 10.1016/j.isci.2022.103928

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-MyoD , Abcam , cat# ab133627; RRID: AB_2890928.

Techniques: Recombinant, Western Blot, Imaging, Multiplex Assay, Methylation, Electrophoresis, Software

Journal: Neural Regeneration Research

Article Title: Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse ☆

doi: 10.3969/j.issn.1673-5374.2012.22.003

Figure Lengend Snippet: Differentiation of adipose-derived stem cells. For myogenisis: (A) immunoflurorescence microscopy revealed that, dko-ADSCs only showed MyoD + cells (12.6 ± 1.5%) and no myotubes. Myotubes appeared on day 28 in dko-microdys-ADSCs (18.0 ± 1.5%) and dko-microdys-β-cat-ADSCs (30.5 ± 2.1%). MHC, desmin, MyoD and myogenin expression in dko-microdys-β-cat-ADSCs was significantly higher than in dko-ADSCs and dko-microdys-ADSCs ( P < 0.05). (B) Reverse trasncription-PCR: the transcription of pax3, pax7, Mif4, Mif5, MHC, myogenin, desmin and MyoD in the two transduced dko-ADSCs groups. For adipogenesis: (C) Oil red O staining. a P < 0.05, vs . dko-ADSCs and dko-microdys-ADSCs. Arrow represents adipogenesis. MyoD: Myogenic differentiation antigen; MHC: myosin heavy chain; ADSCs: adipose-derived stem cells.

Article Snippet: The following primary antibodies were used: MyoD, myogenin, MHC, desmin (MyoD, myogenin, anti-mouse monoclonal antibody; MHC, desmin, rabbit multiple antibody; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and β-cat (goat multiple antibody, R&D Systems Inc. Basel, Switzerland; 1:200 in PBS), and dystrophin (Abcam; 1:400 in PBS).

Techniques: Derivative Assay, Microscopy, Expressing, Staining